Q2 – Week 3 – 19-20
week
Week of 11/18- 11/22
Monday – 11/18 – Academic study hall – period 7 – * Call on Drosophila Lab
period 8
1: DNA Activity – this was done as HW!
a) Reviewed important structural aspects of DNA
b) Students makes 2 dimensional cutouts of 6 nucleotides.
c) Labeled Nucleotides, Direction, and code on the cutout that student keeps as notes.
2. Part 2 – Model building (3 dimensional models)
a) Add to Student DNA presentation (linked last week) by identifying the nitrogenous bases
take picture of 4 nitrogenous bases (models) and identify on the presentation.
Monday – 11/18 – Homework:
Tonights form:
End of Monday…
Tuesday – 11/19 – period 7/8 – LAB!!!
1: Thomas Hunt Drosophila Experiment (#1) – Sex- linked!
What was important about this experiment was that is was the 1st time that an allele was associated on a particular chromosome (the X chromosome). It was the 1st evidence of the chromosomal line of inheritance and although it used parts of Mendelian genetics (dominant and recessive alleles) it also demonstrated that the Law of Independent assortment was not always the case. THERE ARE SCENARIOS where alleles travel on the same chromosome! This is not rare as we have 30,0000 genes in our human DNA and only 23 different chromosomes!
Most genes are linked. Mendel was lucky to pick alleles on pea plants that were not linked so that he could establish the Punnet Squares and the laws of probability that result from unlinked alleles.
2. Finish DNA activity –
a) Build the DNA molecule!
Work on DNA Activity – Part 2 – Model building (3 dimensional models)
Your work on the DNA Activity will be completed on the the linked Presentation that you have been using since last Thursday.
DNA Activity Instructions: IT is DUE !!! No more time in class!!!
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1: Title Page, with your name: slide page 1 2: 1st Notes page:
General Label Nucleotide: nucleotide, sugar, phosphate and nitrogenous base on slide page 2
3. 2nd Notes page: Pyrimidines, Purines, Name of the nucleotides based on nitrogenous bases on slide page 3
4: 2nd Notes page with small DNA fragment(6 nucleotides total with 3 on each complimentary strand):
Illustrate and label: phosphodiester bonds, identified all nucleotides (Adenine, Thymine, Guanine, Cytosine),
On slide 4 5′ or 3′ direction of DNA fragment, correct H-Bonds with dots between the nucleotide
pairs, Make sure your nucleotide pairs are correct and your orientation of your
complimentary strands.
Write the DNA’s fragment code for both strands: example: 5′ ATA 3′
3′ TAT 5′
Part 2 : 3D DNA model building.
5: Take a photo of the 4 nucleotides of the model kit of your color and
Label: nucleotide name, Purines, Pyrimidines and place carbons, Nitrogens and oxygens on top of the models
On slide 5
6. Take a photo of the Sugar Phosphate backbone and
Identify and label: the phosphate, and deoxyribose (sugar).
On slide 6
Number the carbons on the sugar.
7. Try to Build the SAME 6 nucleotide DNA segment that you created in step 4 above.
8. Take a Photo of the DNA segment and label the image with arrows and labels with the same list from step 3.
On slide 7
9. Connect your segment with others in your group or class to create a very large DNA segment.
a) Take a photo and label the direction on a new slide On slide 8
b) and Write the DNA Code for both strands on the slide
10. Make sure you have Titled every page.
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11/19 – Tuesday – Homework: THE DNA ACTIVITY IS DUE tomorrow after 7th period!
1. Make another submission to Last nights form.
2: Make sure your DNA activity is complete for grading (will count as a lab).
Please take note of the DNA Activity Instructions posted above.
Edgar and Evan: I helped you assemble your DNA strand. Here is the picture of it:
Please use this image for your second to last slide.
3: Watch the lecture on the Pedigree Diagrams and take notes using the
Pedigree Diagram Notes 1718.pdf worksheet that I gave you in class or posted below. If you cannot print then just do so with a piece of paper.
4: Complete the Pedigree autosomal and linked.pdf worksheet and review with the key.
5: Complete the Pedigree Diagram Form
with the worksheets below
Pedigree Diagram Lecture:
Pedigree Diagram Form:
End of Tuesday..
Wednesday – 11/20 – period 7 – Academic study hall
1. Final assembly of the complete Class DNA molecule with its code. You will use this last picture for the last slide.
2. Resubmit pedigree diagrams (last nights form)
– period 8
DNA Replication: Note taking!!!
1. Whiteboard activity – Draw the replication fork in DNA Replication
a) Identify and label the template strands with 5′ and 3 ‘ ends.
b) Draw in with a different color primers and the daughter strand.
c) Identify the Leading and Lagging strand.
d) Identify the Okazaki fragments and direction of nucleotide addition by DNA polymerase III
Here is a picture of my whiteboard:
11/20 – Wednesday – Homework
1: Please make a second submission to last nights form.
2: Please view the three college lectures to answer the questions from the form below.
THE FORM WILL DIRECT YOU TO EACH VIDEO!
College Lecture 1:
College Lecture 2:
College Lecture 3:
Tonights form:
End of Wednesday…
11/21 – Thursday – period 7/8 Lab!
*Review the idea of linked genes from Wednesday..
1. Take picture of the Class DNA, made from all the student group DNA segments.
This the last slide of the DNA activity. You also need the Code!!!!
2. Sordaria Fimicola Lab – begin – note taking in Marble Notebooks!
3. Set-up the Sordaria Firmicola agar plates
We are culturing 2 types of fungi (decomposer), Tan – mutant and Brown – wild type. We put them in four alternating quadrants in the petri dish so that the mutant and wild type will sexually cross to make hybrids. We then placed the petri dishes in the incubator (38 degrees Celsius) for about a week so that we can take samples of the hybrids and view the hybrids ascospores (group of eight spores). The color of the fungi is due to the color of the ascospores and we will determine the crossover percentages by viewing the pattern of the ascospores (tan or brown). I will explain this completely again when we get to the next phase in this lab.
4. “VOICE OVER PROJECT” – DNA replication
“VOICE OVER PROJECT” – DNA replication
Your group will make a story board of all the steps of DNA Replication.
Please place a screenshot of the video into a new page of the Google Doc and add as many screenshots of the animation as necessary to completely and thoroughly describe the video using captions that you will synthesize from your text book.
Link to video file for “VOICE OVER PROJECT” – DNA replication”
You will need to use your text pages 313 – 318 to help learn all the important steps of DNA replication. Whenever you use an important vocab word please bold it and add its definition below the caption. Presentation counts and well as content.
So you will need to make screenshots of the video that you will open on your computer. These screen shots will be placed into the Google Doc THAT I SHARED WITH YOUR GROUP. Each screenshot will have words below it that you will makes with your group (using the textbook) to describe the step in the replication of DNA. Make as many as slides as possible to describe the entire process.
Work together by adding comments and chatting by clicking comments above. Do not let the LVP off the hook. Get them involved. Everyone is responsible for understanding the content. This graded project will be based on presentation, content, and accuracy. Humor is appreciated but accuracy is still important.
Good Luck!
Look for the link for the google doc for each group THAT I JUST SENT Today. It is already linked by each lab group. Check your private email (gmail) account!
11/21 – Thursday – Homework
1. Please make a second submission to Wednesday nights form.
2. Complete the Storyboard project as a group if you have not already done so in class.
3. Please read the article below from Newsday 2 years ago.
4: Please view my lecture on DNA replication to the lecture below:
5: Please read the article on Telomeres.
6: Answer the questions on the DNA Duplication form.
*Be prepared to have only 1 submission!
Newsday article: – You could also read it online with this LINK:
Mr. Grodski Lecture on DNA replication (including DNA shortening) :
Article on Telomeres:
Telomeres:
A very interesting article on the current research in Telomeres.
Tuesday’s night homework form:
End of Thursday..
Friday – 11/22 – Academic study hall – period 7
1. Take picture of class DNA model and code it and add it to the last page of the DNA activity.
2. Resubmit last night’s form – DNA Duplication form
3. Complete the DNA activity.
4. DNA Duplication Voice Over Activity.
Please download the following file:
Blank DNA Replication video VOICE over.mp4
Download
Download
In this activity you will bring your slides that you made from your Google Docs with their corresponding captions to your Lab Group. You will assimilate the information into your team in order to create an actual voice-over that is appropriate and in sync with the animation through Imovie on your Laptops. Your Team with create a movie with this voice-over added and will air drop it to me.
11/22 – Friday Homework –
1. Please make a second response to the DNA duplication form.
2. Fix your DNA Replication Storyboards!!
Most groups neglected to read the directions and did not include a vocabulary section!
This excerpt is from the instructions above (from Thursday) and from the shared doc:
“You will need to use your text pages 313 – 318 to help learn all the important steps of DNA replication. Whenever you use an important vocab word please bold it and add its definition below the caption. Presentation counts and well as content. “
3. Make sure that the Brine Shrimp Lab is completed. I am going to grade it soon. I will not be reading labs prior to grading. You should know what I expect and those guidelines were posted at the end of week 2 in the second quarter.
4. Please complete the form below on Morgan’s second ground breaking
Drosophila experiment. Do not lose sight that what we are studying this weekend has
everything to do with replicating DNA (in the S1 of the life cycle of the cell) before meiosis!
End of week 3!!!!