Q2 – Week 6 – 19-20
Week of 12/9 – 12/13
12/9 – Monday – period 7 – Academic Study Hall
period 8 –
1. CBS – 60 minutes – Rewind –
2. FAST PLANT LAB!
3. Hand back Gene to Protein Form 2 –
a) Connections of Alternative RNA Splicing – nematodes and us
b) having introns allows for an increased opportunity for Crossover to unlink for genes.
This might might provide more terrain from crossovers without
interrupting coding sequences.
c) exon shuffling – Exons could shuffle from one chromosome to another in crossover and with
Alternative RNA Splicing this might provide new combinations of exons and thus possibly new proteins that may have lead to evolutionary advantages.
Transcription through Translation:
Translation:
3D animation:
12/9 – Monday Homework
1: Gene to Protein DRAW
YOU have a Google Presentation that has been emailed to you (or the link was email). Please follow the directions which are also in the Google presentation:
Please draw diagrams that illustrate the following stages of Translation:
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THe mRNA interacts with the rRNA of the ribosome to initiate translation at the start codon.
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The sequence of nucleotides on the mRNA is read in triplets called codons.
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tRNA brings the correct amino acid to the correct place on the mRNA.
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The amino acid is transferred to the growing peptide chain.
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The process continues along the mRNA until a stop codon is reached
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The process terminates by the release of the newly synthesized peptide/protein.
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Make sure that you include the P, A, and E sites
Draw these diagrams on separate piece of paper and then take a picture using your computer and paste it into this Google Presentation. You can accomplish this many ways but I need you to draw diagrams that illustrate the list above. MAKE SURE YOU ANNOTATE YOUR Diagrams. Use the text page 338 – 343 or the videos I posted that may help with the visuals.
These videos posted above and below might be helpful:
You are expected to understand to fully understand these video’s by tomorrow.
End of Monday!
12/10 – Tuesday – period 7/8
1: Studio 230 – Translation activity
Today’s Translation Studio:
Are you guys the GOAT?
2: Types of Mutations – (substitutional)
12/11 – Tuesday Homework: FAST PLANT LAB CAN BE DUE NEXT MONDAY!
1: I NEED THOSE GROUPS THAT HAVE NOT AIR DROPPED ME THEIR DNA PROJECTS TO DO
SO ASAP!!
2: ALSO THERE ARE GROUPS WHO DID NOT FINISH THEIR mRNA Activity Still. NEEDS to be
completed by this Friday.
3: Complete Form Below:
End of Tuesday!
12/11 – Wednesday – period 7 – Academic Study Hall
1. second submission of last night form – Due in class
period 8 –
1: Mutations – completed the Mutation worksheet together
Substitutional Mutations:
Missense –
Silent –
Nonsense –
Reading Frame Mutations:
Insertions –
Deletions –
12/12 – Wednesday Homework:
1. Please watch the video below and complete the form:
If the video is blocked please use the following link to view the video:
End of Wednesday!
12/13 – Thursday – period 7/8 –
I am giving you back a study hall today as I will use it for our 1st biotechnology lab.
1. Lac Operon discussion –
2: trp operon opening discussion.
3. Review GOT Lactase – HW
4. WHERE HAVE WE talked about a mutation in a regulatory protein or a switch?
Where has there been a huge change in phenotypes due to a small number of mutations?
Could operators and regulatory proteins in an operon be the root of these
examples:
a)
b)
5. Positive Control gene regulation – with worksheet.
12/12 – Thursday – Homework – Fast Plant Lab 1 – due Monday 12/16
1. You should have the following pages completed for tomorrow.
Pages: 2,3,4 (Skip page 8 questions. Put a Y through page 8) and page 12.
2. Please watch both short Lectures from a tall teacher.
3. Please FILL OUT THE FORM below. You may need the text book!
Lecture 1:
Lecture 2:
12/13 – Friday – period 7 – Academic Study Hall
period 8 –
I will be using the Academic Study hall for today for our
Gel electrophoresis Lab
1. Crime scene Lab – pouring the gels/intro
2: Micropippeting restrictive enzymes
3: incubating DNA and restrictive enzymes
4: Loading Gels
5: Gels are run in the afternoon 30 min at 100Volts
DNA Fingerprinting Lab period 6:
Standard Suspect #1 Suspect #2 Suspect #3 Suspect #4 Suspect #5 Crime Scene
Molecular Gavin Jack Morgan D Evan Kristina
Ladder
Who is guilty?
Emma and Theresa: |
Edgar and Evan |
Christie , Morgans |
Kristina, Gavin, Jack, Kade |
Max and Dan |
If you look at lane 4 (suspect 3) and lane 7 (CS), especially in Kristina’s group gel then it would be wise for Morgan D. to get a lawyer!
12/14 – Friday Homework:
1. Complete the FAST Plant (Lab 1).
Please include recombinant information.
Please include Genetic Drift information – allele frequency change from one generation to the next.
Fast Plant: Lab
P1: (this was the parents of our original seeds) : ANL/ANL YGR/YGR x anl/anl ygr/ygr
Purple Stems Green Leaves Green stems yellow leaves
F1: (this was the genotype of the seeds we first planted) : ANL/anl YGR/ygr x ANL/anl YGR/ygr
Carriers of the parental alleles
Purple Stems Green Leaves Purple Stems Green Leaves
F2: (this was the generation of the seeds of OUR second planting: There will be 4 different phenotypes:
Parental: Purple Stems, Green Leaves
Purple Stems, Yellow Leaves
Green Stems, Green leaves
Parental: Green Stems,Yellow leaves
When doing the % recombinants, remember that we are determining the parental phenotypes of the ORIGINAL Parental Generation (P1). The dihybrid cross is a cross of the carriers of the parental alleles.
Because both parents are undergoing recombination we will divide our percent recombinants by 2 to get the corrected percent recombinants (which will now represent the morgan unit on a gene map).
I will do a separate test cross with (homozygous recessive plants with F2) next year to provide better data!
END of Week 6!