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Q2 – Week 6 – 20-21

week 6

Week of 12/21 – 12/23- 
 
*Please REFRESH this Page every time you view!!!
The 5 day – A, B, C, D cycle looks like this:
                                                       Day                      Period
                                                                             7                                  8                              
                
    Monday                 In class:         A         Academic Study               AP BIOLOGY                  
                                   Remote:                       Academic Study                   AP BIOLOGY              
 
                                         In class:         B         AP BIOLOGY                   AP BIOLOGY                      
                                                          Remote:                    AP BIOLOGY                 AP BIOLOGY           
 
                                            In class:              C         AP BIOLOGY                   AP BIOLOGY              
                                   Remote:                     AP BIOLOGY                AP BIOLOGY       
 
                                        In class:          D         Academic Study               AP BIOLOGY  
                                   Remote:                     Academic Study             Academic Study     
                 
 
This week’s 3 day Schedule:   I = In person,  R = Remote
                                                                                   
                                                                                                      
12/21  – Monday – “A” Day   – period 7A, 8A – I  7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
                                                       –period 7A, 8A -R  7 (A) REMOTE INSTR –  ASH  / 7(C) 8(A,C) 20-21 REMOTE INSTR
 
 
12/22 –  Tuesday  –  “B” Day   –period 7B, 8B– I   7(B) 8(B,D)  AP BIOLOGY – (double period Lab)
                                                         – period 7B, 8B -R  7(B) 8(B,D)  AP BIOLOGY – REMOTE INSTR
 
 
12/23  – Wednesday – “C” Day  – period 7C, 8C -I     7(C) 8(A,C)  AP BIOLOGY – (double period Lab)
                                                            – period 7C, 8C –R   7(C) 8(A,C)  AP BIOLOGY – REMOTE INSTR
 
12/21  – Monday – “A” Day   – period 7A, 8A – I  7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
                                                       –period 7A, 8A -R  7 (A) REMOTE INSTR –  ASH  / 7(C) 8(A,C) 20-21 REMOTE INSTR
The RED team is Remote Today.  Please move to the Remote Instruction Page. 
 
1.  Culture the Sordoria Fimicola Fungi – 
 
 2. mRNA activity begin – build DNA template strand. I have listed the entire set of instructions to begin the  
                                                      activity. 
 
You may need the digital file to see if your strand is correct!  You are acting like a CRISPER/Cas- 9 enzyme cutting and adding DNA. 
 

mRNA activity student copy .pdf

 
Please Follow the Instructions below to complete the mRNA activity:
 
Please find your work in the back lab table under the light.
 
1. Complete the Template strand of the DNA by writing the correct complimentary code below the coding strand.
 
2.  Connect a single stranded blank strand that is almost as long your DNA strand. This will be eventually be your mRNA but for now it will be your pre-mRNA (or primary transcript)
This blank strand sheet that needs to be cut out and assembled are on the first desk next to the scissors and tape box.
 
3.  Mark on your DNA strand with a highlighter the promoter region and the termination sequence. If you forget what these small bits of code are for each then grab the text in Chapter 17 and look it up.  
 
The promoter region is telling where to start represents the INITIATION step.
 
*When you look for this code it is helpful to look and the coding strand (The printed code above the Template strand that you filled in).  The coding strand will reveal what the mRNA Strand will look like EXCEPT THAT thymine will be Uracil!
 
4.  Lay your blank RNA strand along the Template Strand (that you filled out) and start making the RNA primary transcript from 15 or so UTR’s  downstream from your promoter region.  It must be in front of the start CODON: AUG which will look like ATG on the Coding strand!   So start coding the mRNA strand remembering to use Uracil instead of  THymine.  
 
Writing the code on the single strand mRNA is the ELONGATION step.
 
5. Keep coding until you complete the terminating sequence. At this point your primary transcript (pre-mRNA) will be detached from the Template Strand of the DNA. This is where RNA polymerase detaches from the template strand of the DNA.  
 
Stopping the code and detaching the pre-RNA is the TERMINATION step.
 
6.  Now you have to make your pre-RNA into the processed mRNA that can leave the nucleus of a eukaryote cell and move into another area of the cell that will Translate the mRNA Codons into proteins.  
 
Alteration of the mRNA Ends
 
7. You must modify the ends of this pre-mRNA. You may have look up in the text (page 334) or look below to remember how the ends of the RNA are modified.  You may have to add some blank strands to the ends to make the caps.
This modification facilitates the export of the mRNA out of the nucleus, protects it from it being broken down by enzymes, and helps ribosomes to attach it for Translation.
 
RNA Splicing
 
8. On your pre-mRNA that has its end capped appropriately in step 7, use a highlighter to identify the EXONS.  The Exons are the triplet codons that are expressed.  In your packet you have the code for the entire gene including the start codon and stop codon.  So highlight  ONLY the Triplet CODON in the RNA strand that is in your packet or below.
 

A

U

G

G

U

C

G

A

A

G

U

C

A

U

U

G

C

G

U

C

G

A

A

G

C

G

C

U

G

G

U

C

U

A

A

U

A

A

G

G

G

C

G

U

G

U

U

G

G

U

C

G

A

A

G

U

C

A

U

U

G

U

C

C

C

C

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U

C

G

A

A

A

U

C

C

G

U

U

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G

A

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A

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A

U

U

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C

U

U

A

G

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The code will go in order but it will have many UTR’s (Untranslated REgions) that are not part of the code and these are INTRONS!!!!

After you have highlighted all of the EXONS Physically cut away all of the INTRON in between the start and stop codon and retape (splice) the RNA back together so that there is a continuous code of EXONS from start to stop.  At this point you are acting as snRNA (snurps) in a splicesome!

Your pre-mRNA IS NOW a mRNA and can leave the nucleus to begin Translation.

9.  Roll up your DNA and completed mRNA and place on top of your packet with group names and return this back to the lab area you picked up your work today.

 
12/21  – Monday – “A” Day – Homework – 
 
1. Fast Plant write-up.
2: Gene to Protein DRAW assignment – 
 
YOU have a Google Presentation that has been emailed to you (or the link was email).  Please follow the directions which are also in the Google presentation:
 

Please draw diagrams that illustrate the following stages of Translation:

  1. THe mRNA interacts with the rRNA of the ribosome to initiate translation at the start codon.

  2. The sequence of nucleotides on the mRNA is read in triplets called codons.

  3. tRNA brings the correct amino acid to the correct place on the mRNA.

  4. The amino acid is transferred to the growing peptide chain.

  5. The process continues along the mRNA until a stop codon is reached

  6. The process terminates by the release of the newly synthesized peptide/protein.

  7. Make sure that you include the P, A, and E sites

Draw these diagrams on separate piece of paper and then take a picture using your computer and paste it into this Google Presentation.  You can accomplish this many ways but I need you to draw diagrams that illustrate the list above. MAKE SURE YOU ANNOTATE YOUR Diagrams. Use the text page 338 – 343 or the videos I posted that may help with the visuals.

 
These videos posted above and below might be helpful:
 

YouTube Video

 
End of Monday..
12/22 –  Tuesday  –  “B” Day   –period 7B, 8B– I   7(B) 8(B,D)  AP BIOLOGY – (double period Lab)
                                                        -period 7B, 8B -R  7(B) 8(B,D)  AP BIOLOGY – REMOTE INSTR
 
The Blue team is Remote today. Please move to the Remote Instruction Page.
 
Period 7,8 – 
 
A)   mRNA activity begin – build DNA template strand. I have listed the entire set of instructions to begin the  
                                                      activity. 
 
You may need the digital file to see if your strand is correct!  You are acting like a CRISPER/Cas- 9 enzyme cutting and adding DNA. 
 

mRNA activity student copy .pdf

 
Please Follow the Instructions below to complete the mRNA activity:
 
Please find your work in the back lab table under the light.
 
1. Complete the Template strand of the DNA by writing the correct complimentary code below the coding strand.
 
2.  Connect a single stranded blank strand that is almost as long your DNA strand. This will be eventually be your mRNA but for now it will be your pre-mRNA (or primary transcript)
This blank strand sheet that needs to be cut out and assembled are on the first desk next to the scissors and tape box.
 
3.  Mark on your DNA strand with a highlighter the promoter region and the termination sequence. If you forget what these small bits of code are for each then grab the text in Chapter 17 and look it up.  
 
The promoter region is telling where to start represents the INITIATION step.
 
*When you look for this code it is helpful to look and the coding strand (The printed code above the Template strand that you filled in).  The coding strand will reveal what the mRNA Strand will look like EXCEPT THAT thymine will be Uracil!
 
4.  Lay your blank RNA strand along the Template Strand (that you filled out) and start making the RNA primary transcript from 15 or so UTR’s  downstream from your promoter region.  It must be in front of the start CODON: AUG which will look like ATG on the Coding strand!   So start coding the mRNA strand remembering to use Uracil instead of  THymine.  
 
Writing the code on the single strand mRNA is the ELONGATION step.
 
5. Keep coding until you complete the terminating sequence. At this point your primary transcript (pre-mRNA) will be detached from the Template Strand of the DNA. This is where RNA polymerase detaches from the template strand of the DNA.  
 
Stopping the code and detaching the pre-RNA is the TERMINATION step.
 
6.  Now you have to make your pre-RNA into the processed mRNA that can leave the nucleus of a eukaryote cell and move into another area of the cell that will Translate the mRNA Codons into proteins.  
 
Alteration of the mRNA Ends
 
7. You must modify the ends of this pre-mRNA. You may have look up in the text (page 334) or look below to remember how the ends of the RNA are modified.  You may have to add some blank strands to the ends to make the caps.
This modification facilitates the export of the mRNA out of the nucleus, protects it from it being broken down by enzymes, and helps ribosomes to attach it for Translation.
 
RNA Splicing
 
8. On your pre-mRNA that has its end capped appropriately in step 7, use a highlighter to identify the EXONS.  The Exons are the triplet codons that are expressed.  In your packet you have the code for the entire gene including the start codon and stop codon.  So highlight  ONLY the Triplet CODON in the RNA strand that is in your packet or below.
 

A

U

G

G

U

C

G

A

A

G

U

C

A

U

U

G

C

G

U

C

G

A

A

G

C

G

C

U

G

G

U

C

U

A

A

U

A

A

G

G

G

C

G

U

G

U

U

G

G

U

C

G

A

A

G

U

C

A

U

U

G

U

C

C

C

C

G

U

C

G

A

A

A

U

C

C

G

U

U

G

G

A

G

A

C

A

U

U

G

C

G

C

U

U

A

G

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The code will go in order but it will have many UTR’s (Untranslated REgions) that are not part of the code and these are INTRONS!!!!

After you have highlighted all of the EXONS Physically cut away all of the INTRON in between the start and stop codon and retape (splice) the RNA back together so that there is a continuous code of EXONS from start to stop.  At this point you are acting as snRNA (snurps) in a splicesome!

Your pre-mRNA IS NOW a mRNA and can leave the nucleus to begin Translation.

9.  Roll up your DNA and completed mRNA and place on top of your packet with group names and return this back to the lab area you picked up your work today.

mRNA activity continues:

B) Sordori Fimicola Cultures – 

C) Drosphilia Cross
 
12/22 –  Tuesday  –  “B” Day Homework– 
 
1.  Complete your formal write-up of the FAST PLANT Lab 1 – Due Tomorrow!
2. Please use your book from chapter 17 to complete the multiple choice questions in the form below:
 

Gene to Protein Hw Quiz -2021

 

End of Tuesday.
12/23  – Wednesday – “C” Day  – period 7C, 8C -I     7(C) 8(A,C)  AP BIOLOGY – (double period Lab)
                                                            – period 7C, 8C –R   7(C) 8(A,C)  AP BIOLOGY – REMOTE INSTR
 
The RED team is remote today.  That means the RED team will have to come to school during the holiday break.
 
Period 7,8:
 
1. COMPLETE the mRNA activity.
 
 


12/23  – Wednesday – “C” Day  – Holiday Homework – 
 
 
1. Please check the counter daily and fill the form below to prove your diligence.
 

 

Happy New Year 2021 and leaving year of 2020 concept text on the sea beach

 

End of week 6 and the End of 220!
 
 
NOT UPDATED BELOW~~~~!!!!!
 
 
 

 

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