Q2 – Week 8 – 20-21
week 8
Week of 1/11 – 1/15
PLANT SPINACH FOR PHOTOSYTHESIS LAB – 2 Buckets
Plant Coleus this week!
*Please REFRESH this Page every time you view!!!
The 5 day – A, B, C, D cycle looks like this:
Day Period
7 8
Monday In class: A Academic Study AP BIOLOGY
Remote: Academic Study AP BIOLOGY
In class: B AP BIOLOGY AP BIOLOGY
Remote: AP BIOLOGY AP BIOLOGY
In class: C AP BIOLOGY AP BIOLOGY
Remote: AP BIOLOGY AP BIOLOGY
In class: D Academic Study AP BIOLOGY
Remote: Academic Study Academic Study
This week’s 5 day Schedule: I = In person, R = Remote
1/11 – Monday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
–period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
1/12 – Tuesday – “B” Day –period 7B, 8B– I 7(B) 8(B,D) AP BIOLOGY – (double period Lab)
-period 7B, 8B -R 7(B) 8(B,D) AP BIOLOGY – REMOTE INSTR
1/13 – Wednesday – “C” Day – period 7C, 8C -I 7(C) 8(A,C) AP BIOLOGY – (double period Lab)
– period 7C, 8C –R 7(C) 8(A,C) AP BIOLOGY – REMOTE INSTR
1/14 – Thursday – “D” Day – period 7D,8D – I 7(D) AP BIO ACADEMIC STUDY / 7(B) 8(B,D) AP BIOLOGY
– period 7D,8D – R 7(D) REMOTE INS / 7(B) 8(B,D) AP BIOLOGY REMOTE INSTR
1/15 – Friday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
–period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
1/11 – Monday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
–period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
The Red Team is Remote today. Please move to the Remote Instruction Page.
Period 7,8
1. Review Classwork of Practice Ap Problems
2.pGlo Transformation Lab– Streak the plates
3. Complete the Drosophila Lab with packet. – We did not get to do this today
F1: Offspring – sex the flies / catagorize the offspring/Run Chi squared statistic
1 : Please answer question in PGlo lab packet page 3 (questions 1 – 4) and page 12 (questions 1 – 4)
THis lab Packet is due this Friday!
I will give you a hard copy in class if you did not get one today.
2. Make another submission of the weekend Pglo form. I will have it graded and returned to you.
End of Monday..
1/12 – Tuesday – “B” Day – period 7B, 8B– I 7(B) 8(B,D) AP BIOLOGY – (double period Lab)
-period 7B, 8B -R 7(B) 8(B,D) AP BIOLOGY – REMOTE INSTR
The Blue Team is Remote today. Please move to the Remote Instruction Page.
Period 8,9
1: pGlo Transformation Lab-
a) obtain 2 micro test tubes, one labeled +pGlo and the other -pGlo
Little p means plasmid – small circular DNA of E. Coli.
b) Obtain your starter plates that you streaked yesterday that has been incubating for 24 hours at 37 degrees
Celsius.
c) Use a sterile loop and pick up a single colony and disperse into each tube, +pGLO and -pGlo.
d) I will add 10 micro liters of the pGlo plasmid (engineered sequence) to your +pGLO micro test tube.
e) Incubate the tubes on ice for 10 minutes.
f) label your lids.
g) heat shock, add broth, pippete and streak in the appropriate plates.
f) Stack up the plates, tape and label, then incubate for 24 hours.
2. Complete the Drosophila Lab with packet. –
F1: Offspring – sex the flies / catagorize the offspring/Run Chi squared statistic
1/12 – Tuesday – “B” Day – Homework:
1: Please complete form below based on Chapter 20 of your text:
2: Go back to number 1
End of Tuesday..
1/13 – Wednesday – “C” Day – period 7C, 8C -I 7(C) 8(A,C) AP BIOLOGY – (double period Lab)
– period 7C, 8C –R 7(C) 8(A,C) AP BIOLOGY – REMOTE INSTR
The Red Team is Remote today. Please move to the Remote Instruction Page.
Period 7,8:
1: pGlo Transformation Lab-
a) obtain 2 micro test tubes, one labeled +pGlo and the other -pGlo
Little p means plasmid – small circular DNA of E. Coli.
b) Obtain your starter plates that you streaked yesterday that has been incubating for 24 hours at 37 degrees
Celsius.
c) Use a sterile loop and pick up a single colony and disperse into each tube, +pGLO and -pGlo.
d) I will add 10 micro liters of the pGlo plasmid (engineered sequence) to your +pGLO micro test tube.
e) Incubate the tubes on ice for 10 minutes.
f) label your lids.
g) heat shock, add broth, pipette and streak in the appropriate plates.
f) Stack up the plates, tape and label, then incubate for 24 hours.
1/13 – Wednesday – “C” Day Homework:
1. Start DNA Fingerprinting Lab by answering the following questions on the following pages:
Sorry I had the wrong packet posted here! Now I have the right one posted. Thanks Sintia!
Biorad – DNA Fingerprinting- Student .pdf
If you do not have a hard copy (Red Team) you could just write your responses on a separate piece of paper that you can staple to the packet that I will give you tomorrow.
Page 2: questions 1 – 5
Page 3: questions 1 – 3
Page 4: questions 4, and 5
End of Wednesday..
1/14 – Thursday – “D” Day – period 7D,8D – I 7(D) AP BIO ACADEMIC STUDY / 7(B) 8(B,D) AP BIOLOGY
– period 7D,8D – R 7(D) REMOTE INS / 7(B) 8(B,D) AP BIOLOGY REMOTE INSTR
The Blue team is remote today. THere is no need to link to me today through zoom as you will be doing the same activities that the Red team is doing today. Please move to the homework.
Full REMOTE students: I will do a LIVE stream of the Lab tomorrow so please do the BLUE team homework.
Period 7, 8 –
1. Gather Data for the pGLO lab:
a) Take Pictures of your test plates.
2. Crime Scene lab –
a) pour gels
b) Load gels with DNA from crime scene.
c) Run Gels
1/14 – Thursday – “D” Day Homework
Blue Team: Please add definitions to the vocabulary words to the list below that will be on your Genetics Test.
| Vocabulary words | Text | Vocabulary words | Text |
| Transformation | 306 | codons | 330 |
| bacteriophages | 306 | missense mutation | 334 |
| Virus | 306 | nonsense mutation | 345 |
| Helicase | 314 | Frameshift mutation | 346 |
| Topoisomerase | 314 | operator | 352 |
| single strand binding proteins | 314 | operon | 352 |
| DNA polymerase | 314 | regulatory gene | 353 |
| leading strand | 315 | repressor | 353 |
| Lagging strand | 315 | inducer | 354 |
| nuclease | 318 | RNAi | 365 |
| telomerase | 318 | plasmids | 397 |
| Okazaki fragments | 315 | restriction enzymes | 398 |
| TRanscription | 328 | PCR | 403 |
| TRanslation | 328 | gel electrophoresis | 405 |
| RNA polymerase | 331 | linked genes | 292 |
| promoter | 332 | recombinants | 294 |
| TATA Box | 332 | nondisjunction | 297 |
| INtrons | 335 | polyploidy | 298 |
| Exons | 335 | nucleotide | 87 |
| anticodon | 337 | antiparallel | 88 |
| mRNA | 328 | all marshmallows names in Lucky Charms | 1264 |
| rRNA | 339 | gibberellin | 830 |
| transduction | 562 | conjugation | 562 |
Do not be “foolish”! Review these words!
RED Team:
1. Please complete the following pages in your pGLO lab packet once I give you the 4 test cultures.
Page 13
Page 14
Page 15
Page 16
Do these questions in the order above. My photocopying had the pages out of order.
Do not do page 18 as I said in class..
2. Crime Scene Lab:
a) pour gels
b) Load gels
pGlo Data : RED TEAM: successful Lab!
– pGl0 – LB – pGlo – LB, Amp
+pGlo – LB, Amp +pGlo – LB, Amp, Ara
|
Sophia:  |
Will:  |
|
Jess:  |
Sinta:  |
Crime Scene Lab Gels:
Wells:
ML , CS, Suspect #1 (Sintia), Suspect #2 (Will), Suspect #3 (Jess), Suspect #4 (Sophia), Suspect #5 (Mr.G)
|
Sophia  |
Will:  |
Jess |
Sintia:  |
Jess, I think its time to get an attorney…
Jessica’s and Sintia’s gel are the most conclusive!
End of Thursday!
1/15 – Friday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
–period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
The RED Team is Remote today. Please move to the Remote instruction page.
RED Team: Please add definitions to the vocabulary words to the list below that will be on your Genetics Test.
| Vocabulary words | Text | Vocabulary words | Text |
| Transformation | 306 | codons | 330 |
| bacteriophages | 306 | missense mutation | 334 |
| Virus | 306 | nonsense mutation | 345 |
| Helicase | 314 | Frameshift mutation | 346 |
| Topoisomerase | 314 | operator | 352 |
| single strand binding proteins | 314 | operon | 352 |
| DNA polymerase | 314 | regulatory gene | 353 |
| leading strand | 315 | repressor | 353 |
| Lagging strand | 315 | inducer | 354 |
| nuclease | 318 | RNAi | 365 |
| telomerase | 318 | plasmids | 397 |
| Okazaki fragments | 315 | restriction enzymes | 398 |
| TRanscription | 328 | PCR | 403 |
| TRanslation | 328 | gel electrophoresis | 405 |
| RNA polymerase | 331 | linked genes | 292 |
| promoter | 332 | recombinants | 294 |
| TATA Box | 332 | nondisjunction | 297 |
| INtrons | 335 | polyploidy | 298 |
| Exons | 335 | nucleotide | 87 |
| anticodon | 337 | antiparallel | 88 |
| mRNA | 328 | all marshmallows names in Lucky Charms | 1264 |
| rRNA | 339 | gibberellin | 830 |
| transduction | 562 | conjugation | 562 |
Do not be “foolish”! Review these words!
Blue Team:
1. Please complete the following pages in your pGLO lab packet after give you the test cultures.
Page 13
Page 14
Page 15
Page 16
Do these questions in the order above. My photocopying had the pages out of order.
2. Crime Scene Lab:
a) pour gels
b) Load gels
pGlo Data : RED TEAM: successful Lab!
– pGl0 – LB – pGlo – LB, Amp
+pGlo – LB, Amp +pGlo – LB, Amp, Ara
|
Sarah:
 |
Grace:  |
|
Charlie:  |
Conner:  |
Crime Scene Lab Gels:
Wells:
ML , CS, Suspect #1 (Connor), Suspect #2 (Grace), Suspect #3 (Sarah), Suspect #4 (Charlie), Suspect #5 (Mr.G)
|
Sarah:  |
Grace:  |
|
Charlie:  |
Conner:  |
Sarah I would think its time to get an attorney!!
Connor, I would think of getting another forensics lab.
Finished gel reveal: Charlie!
1/15 – Friday – “A” Day Homework –
1. Complete the Genetics Test Homework list with definitions from your text using the vocabulary words posted above. You will take the Vocabulary part of the test next Wednesday/Thursday.
2. Complete the pGlo Lab packet up to page 16. I will collect Tuesday and Wednesday next week.
3. Complete the following pages in the Crime Scene Lab:
pages 2,3,4, 12,
page 18 but please only complete the following questions from page 18:
You need a gel that has excellent clarity to answer some of these questions. Use any gel from
either class if you own gel that I posted here does not have good clarity.
questions 1,3,4,5,6,7, 8 (use one of the gels posted instead)