Q3 – Week 5 – 20-21
week 5
Week of 3/8 – 3/12 –
*Please REFRESH this Page every time you view!!!
The 4 day – A, B, C, D cycle looks like this:
Day 7 Period 8
In class: A Academic Study AP BIOLOGY
Remote: Academic Study AP BIOLOGY
In class: B AP BIOLOGY AP BIOLOGY
Remote: AP BIOLOGY AP BIOLOGY
In class: C AP BIOLOGY AP BIOLOGY
Remote: AP BIOLOGY AP BIOLOGY
In class: D Academic Study AP BIOLOGY
Remote: Academic Study Academic Study
This week’s 5 day Schedule: I = In person, R = Remote
3/8 – Monday – “C” Day – period 7C, 8C -I 7(C) 8(A,C) AP BIOLOGY – (double period Lab)
– period 7C, 8C –R 7(C) 8(A,C) AP BIOLOGY – REMOTE INSTR
3/9 – Tuesday – “D” Day – period 7D,8D – I 7(D) AP BIO ACADEMIC STUDY / 7(B) 8(B,D) AP BIOLOGY
– period 7D,8D – R 7(D) REMOTE INS / 7(B) 8(B,D) AP BIOLOGY REMOTE INSTR
3/10 – Wednesday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
– period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
3/11 – Thursday – “B” Day –period 7B, 8B– I 7(B) 8(B,D) AP BIOLOGY – (double period Lab)
-period 7B, 8B -R 7(B) 8(B,D) AP BIOLOGY – REMOTE INSTR
3/12 – Friday – “C” Day – period 7C, 8C -I 7(C) 8(A,C) AP BIOLOGY – (double period Lab)
– period 7C, 8C –R 7(C) 8(A,C) AP BIOLOGY – REMOTE INSTR
3/8 – Monday – “C” Day – period 7C, 8C -I 7(C) 8(A,C) AP BIOLOGY – (double period Lab)
– period 7C, 8C –R 7(C) 8(A,C) AP BIOLOGY – REMOTE INSTR
The Red Team is Remote today.
Zoom Link:
Topic: AP Biology – 03.08 Periods 7, 8
Time: Mar 8, 2021 12:00 PM Eastern Time (US and Canada)
Join Zoom Meeting
https://us02web.zoom.us/j/89058091702?pwd=bjNURFhZdytZb0xRc3JCZTN0RmdPdz09
Meeting ID: 890 5809 1702
Passcode: bc9Sc0
One tap mobile
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Period 7, 8
We will begin the Photosynthesis lab by performing chromatography to separate the pigments of the spinach leaves that are in the photosystems.
1: Photosynthesis Lab part A:
Separating the pigments in the photosystem II an I
Chromatography/spectrophotometry
*USE Chromatography solvent 9:1 (90% Petroleum Ether, 10% actetone) WARDS
Photosynthesis pigments Lab – complete data collection
Chromatography Demo:
Part 1 – Chromatography, separation of the pigments, and identification.
Todays Data From Class Today:
I am posting 2 images form your spectrophotometer today. The first one has your data with each pigment separated. The second one that I ran on your spectrophotometer with spinach leaves that you left on the table soaked with the solvent. This represents ALL the pigments collectively and should look similar to the image with the pigments separated. This should be discussed.
Please use both graphs in your data section.
ALL PIGMENTs (mixed together) absorption graph:
Click on it and then save to your computer.
**Get a good start on this!! IT is not expected to be done by tomorrow.
*Remember the purpose to identify the pigments in the photosystems that help capture photons of light from the sun to excite electrons to higher energy states. Use you lab packet to help.
You must have the following:
You are to do this in Google Slides like we did for the Sordari Firmicola Lab EXCEPT everone will be doing their own slide up.
1. Title Slide
2. Background slide(s) – Do no make these slide all words or all pictures. Use this presentation
format to use more visuals but with explanations. Be creative, using the
concepts of the scientific method.
3. Objectives, Question, and Hypothesis – There is no real hypothesis in this lab.
There are important objectives:
1. Identify as many of the pigments in the photosystems are possible. The major players are
Carotene –
Xanthophyll –
Chlorophyll a –
Chlorophyll b –
a) You will Separate and identify the pigments in the spinach.
You will need to identify each band in the chromatograph by their
particular absorption spectrum, that when added together looks like the Total
Pigments absorption spectrum that we did earlier in the week (in slide 9 ) of my
presentation.
b) Using the Appendix A in your lab OR ANY OTHER RESOURCES and your lab file posted below you can
identify the pigment that was isolated in each band, based on absorption peaks.
2. Procedure – comprehensive list of steps so that anyone can follow.
3. Materials-
4. Data – Graphs from the logger pro files that are posted above WITH YOUR pictures of YOUR chromatograph.
I want to see you identify each line on the image posted above for each pigment you separated.
Please use the lab packet given out or the internet to find graphs from spectrophotometers to help
identify each pigment.
5. Results –
6. Conclusion – Do not be vague but to the point.
Explain your results. Make a leap. Fully explain any errors or limitations of the lab.
7. SOURCES
Full Remote Students Data: click and save to your computers
The other pics like the chromatograph layers will be posted as soon as the student send them to me.
3/8 – Monday – “C” Day – Homework:
1. RED TEAM – Please make another submission to this weekends Form. The 1st submission will be graded and sent you for review.
2. RED TEAM – Please complete the Ecology Form 2 below using your textbook.
Ecology Form 2:
End of Monday..
3/9 – Tuesday – “D” Day – period 7D,8D – I 7(D) AP BIO ACADEMIC STUDY / 7(B) 8(B,D) AP BIOLOGY
– period 7D,8D – R 7(D) REMOTE INS / 7(B) 8(B,D) AP BIOLOGY REMOTE INSTR
The Blue team is remote today!
ZOOM Link:
Topic: AP Biology 03.09 Periods 7,8
Time: Mar 9, 2021 12:00 PM Eastern Time (US and Canada)
Join Zoom Meeting
https://us02web.zoom.us/j/88140736169?pwd=eTJ0bUZ3UzU2bWpOcGRQMEtWalZNUT09
Meeting ID: 881 4073 6169
Passcode: Le9tD3
One tap mobile
+16465588656,,88140736169#,,,,*492921# US (New York)
period 7,8:
1: Photosynthesis Lab part A:
Separating the pigments in the photosystem II an I
Chromatography/spectrophotometry
*USE Chromatography solvent 9:1 (90% Petroleum Ether, 10% actetone) WARDS
Photosynthesis pigments Lab – complete data collection
Chromatography Demo:
Part 1 – Chromatography, separation of the pigments, and identification.
Todays Data From Class Today:
I am posting 2 images form your spectrophotometer today. The first one has your data with each pigment separated. The second one that I ran on your spectrophotometer with spinach leaves that you left on the table soaked with the solvent. This represents ALL the pigments collectively and should look similar to the image with the pigments separated. This should be discussed.
Please use both graphs in your data section.
ALL PIGMENTs (mixed together) absorption graph:
Click on it and then save to your computer.
Pictures of the Chromatograph Layers:
Coming..
**Get a good start on this!! IT is not expected to be done by tomorrow.
*Remember the purpose to identify the pigments in the photosystems that help capture photons of light from the sun to excite electrons to higher energy states. Use you lab packet to help.
3/9 – Tuesday – “D” Day Homework –
1. BLUE TEAM – Complete the Ecology Form 2 posted yesterday AND make sure you have made a total of 2 submissions for Ecology Form 1 that was posted last Friday.
2. BLUE TEAM – Continue working on the Pigment Lab “slide up”. Please cut and paste your work into the presentation I linked to you today. I forgot to do so yesterday.
3. RED TEAM – Begin your lab write-up (SLIDE up) on the presentation that I linked to you today.
End of Tuesday
3/10 – Wednesday – “A” Day – period 7A, 8A – I 7(A) AP BIO ACADEMIC STUDY(ASH) / 7(C) 8(A,C) AP BIOLOGY
– period 7A, 8A -R 7 (A) REMOTE INSTR – ASH / 7(C) 8(A,C) 20-21 REMOTE INSTR
The Red Team is remote today – Please work on your slide- up of the Pigment Lab AND or Start the homework.
Zoom Link:
Topic: AP Biology – 03.10 periods 7,8
Time: Mar 10, 2021 12:00 PM Eastern Time (US and Canada)
Join Zoom Meeting
https://us02web.zoom.us/j/89163196510?pwd=VzFuS2lXRjJ2RTQ4bTlrTy91eFlMQT09
Meeting ID: 891 6319 6510
Passcode: d4jqZi
One tap mobile
+16465588656,,89163196510#,,,,*576092# US (New York)
1. The Rate of Photosynthesis Lab begins
a) gathered control data
DO NOT USE SOAP SOLUTION TO SINK THE disks!!!
Use the .2 % solution ( 2 grams in 1000 ml of water).
2. began thinking about what independent variables you want to test.
3/10 – Wednesday – “A” Day Homework:
1: Continue working on the slide-up of the Pigment Activity. Your conclusion should include the identification of the pigments. The slide- up is due Friday 3/12.
2. Make another submission on the Ecology Form 2 (if needed).
3: Complete Ecology Form 3 with your textbook:
End of Wednesday.
3/11 – Thursday – “B” Day – BOTH TEAM’s Present!
ZOOM Link:
Topic: AP Biology – 03.11
Time: Mar 11, 2021 12:00 PM Eastern Time (US and Canada)
Join Zoom Meeting
https://us02web.zoom.us/j/82625855007?pwd=dHBBT1E4OWVEMVk0VUZ1dlJWd2YxUT09
Meeting ID: 826 2585 5007
Passcode: m7N0jZ
One tap mobile
+16465588656,,82625855007#,,,,*570501# US (New York)
Note to me: DO NOT USE SOAP SOLUTION TO SINK THE disks!!!
Use the .2 % solution ( 2 grams in 1000 ml of water).
1. The Rate of Photosynthesis Lab continues..
*Do not use any soap!!!
*Also use syringes medium to small to sink spinach disks. Stay away from vacuum
chamber.
a) Create a Plan of your unique study.
b) ET 50 explanation
c) start gathering data for guided inquiry lab
Today you need to decide on your independent variable:
Here are some possibilities:
A: Brightness – Distance of the light source.
B: Color or wavelength of light – cellophane filters
C: the amount of CO2 in water – different concentrations of the Bicarbonate solution
D: Organic vs Non-Organic –
E: pH – use different CO2 solutions with varying pH
F: YOUR IDEA?????????????????????????????????
Also I tested the cellophane filters and the following light traveled through each of the filters.
Here are the pictures of the wavelengths of the light that traveled through the films:
It appears that the cellophane filters did filter some the light but it did reemit the color of the cellophane.
Complete spectrum: No filters
Clear cellophane: Some light from light bulb is filtered.
Blue cellophane: A lot of red is filtered out but a lot of Blue is emitted.
Green Filter: Some blue is filtered and some red is but green is emitted.
Red Filter: A lot of greens are filtered but a lot of red is emitted.
3/11 – Thursday – “B” Day – Homework:
1. Slide- up of the Photosynthesis Pigments Lab in the presentation is due Monday 3/15.
2. Please make another submission to the Ecology 3 Form (last nights form).
3. Please view the videos below and answer the form below:
Video 1: Overview of Cell Structure: It will not play from this page. Click on video to play in youtube.
Video 2 : Endoplasmic Reticulum:
Video 3: Protein Trafficking:
Tour of the animal cell:
Cell Anatomy Form:
End of Thursday..
3/12 – Friday – “C” Day – All teams!
ZOOM Link:
Topic: AP Biology 03.12 – Periods 7,8
Time: Mar 12, 2021 12:00 PM Eastern Time (US and Canada)
Join Zoom Meeting
https://us02web.zoom.us/j/85395559027?pwd=bE9rY1U3TzZZTmxCa2JiMGplcUFLUT09
Meeting ID: 853 9555 9027
Passcode: miMj4W
One tap mobile
+16465588656,,85395559027#,,,,*141751# US (New York)
Video of the data collection:
1. Everyone – Make another control run of your data
a) complete your trials using the independent variable of your choice.
2. We will be generating ET50 values from each trial.
Make sure you have Logger pro installed on your computers. Please open Self – Service and download from SCHOOL logger pro.
3: Create your graphs and include the procedure and data into your labs.
You will need graphs to obtain the ET 50 values. Remember that we are investigating RATE = how fast is photosynthesis occurring and thus we need to use the SLOPE of these initial graphs to obtain the ET 50’s, just like we used slope in the Catalase lab. Once you obtained your ET 50 value from each TRIAL then you need to create another graph (bar maybe) that illustrates the possible trends that the independent variables may be influencing the dependent variable.
What else would be necessary when using graphs ? HMMM….error bars..
4. You should have the Materials and Procedure section and the DATA section completed this
weekend. YOU should start making tables or graphs.
New ET50 calculation video:
HMMM..
How to make error bars in Excel. I am showing you how thus is done for a line graph but how would you could apply the same technique with a Bar Graph.
I have posted a copy of the following file that you can just manipulate to get
your tables and graphs from
3/12 – Friday – “C” Day – Homework:
1. Make another submission to last nights form.
2. Complete the pigments lab – slide up – Due Monday.
3. Start your Rate of Photosynthesis Lab:
Title Page –
Background –
Procedure and materials –
Data – You need to have all your data tables, and graphs complete
c
Results –
The only part that should be left is your analysis and or conclusion.
End of week 5!